CEBPZ Polyklonaler Antikörper

CEBPZ Polyklonal Antikörper für IF, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 25612-1-AP

Synonyme

CBF, CBF2, CCAAT binding factor, CEBPZ, HSP CBF, NOC1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 25612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 25612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 25612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 25612-1-AP (CEBPZ Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 25612-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 25612-1-AP (CEBPZ antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

25612-1-AP bindet in WB, IF, ELISA CEBPZ und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEBPZ fusion protein Ag11003
• Vollständiger Name: CCAAT/enhancer binding protein (C/EBP), zeta
• Berechnetes Molekulargewicht: 1054 aa, 121 kDa
• Beobachtetes Molekulargewicht: 120-130 kDa
• GenBank-Zugangsnummer: BC034475
• Gene symbol: CEBPZ
• Gene ID (NCBI): 10153
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEBPZ (CCAAT/enhancer binding protein zeta) belongs to the CBF/Mak21 family, it plays a role in cellular response to environmental stimuli through a transcriptional process that involves heat shock factors, conserved DNA elements (heat shock elements or HSEs) and CCAAT boxes. The protein acts as a DNA-binding transcriptional activator and regulates the heat-shock protein 70 (HSP70) promoter in a CCAAT-dependent manner.

Publikationen

Species	Application	Title
human	WB	Cancer Sci; CEBPγ facilitates lamellipodia formation and cancer cell migration through CERS6 upregulation.; Authors - Hanxiao Shi
mouse	WB	Mol Cell Proteomics; Characterization of R-Loop-Interacting Proteins in Embryonic Stem Cells Reveals Roles in rRNA Processing and Gene Expression.; Authors - Tong Wu