CEL Polyklonaler Antikörper

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• KD/KO Validated

CEL Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15384-1-PBS

Synonyme

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  WB analysis of human liver using 15384-1-AP (same clone as 15384-1-PBS)

  human liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 15384-1-AP (same clone as 15384-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse liver using 15384-1-AP (same clone as 15384-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 15384-1-AP (same clone as 15384-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15384-1-AP (CEL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15384-1-AP (CEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and TRITC-Goat anti-Rabbit IgG, CoraLite® Plus 647 Glucagon antibody (CL647-15954, Magenta), CoraLite®488 INS antibody (CL488-66198, Clone: 4B6A7, green). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human pancreas cancer using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using CEL antibody (15384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), INS antibody (66198-1-Ig, Clone: 4B6A7, red). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 15384-1-AP (same clone as 15384-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 15384-1-AP (CEL antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15384-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15384-1-PBS bindet in WB, IHC, IF/ICC, IF-P, Indirect ELISA CEL und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEL fusion protein Ag5262
• Vollständiger Name: carboxyl ester lipase (bile salt-stimulated lipase)
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 67 kDa
• GenBank-Zugangsnummer: BC042510
• Gene symbol: CEL
• Gene ID (NCBI): 1056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.