CEL Rekombinanter Antikörper

CEL Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242761B11

Kat-Nr. : 85081-4-PBS

Synonyme

Bile salt-activated lipase, 242761B11, BAL, Bile salt-stimulated lipase, BSDL

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  WB analysis using 85081-4-RR (same clone as 85081-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85081-4-RR (CEL antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85081-4-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 85081-4-RR (same clone as 85081-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85081-4-RR (CEL antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85081-4-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 85081-4-RR (same clone as 85081-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85081-4-RR (CEL antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85081-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85081-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85081-4-RR against Human CEL were performed. The affinity constant is 2.46 nM.

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Geprüfte Anwendungen

Produktinformation

85081-4-PBS bindet in WB, IHC, Indirect ELISA CEL und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CEL fusion protein Ag5262
• Vollständiger Name: carboxyl ester lipase (bile salt-stimulated lipase)
• Berechnetes Molekulargewicht: 79 kDa
• Beobachtetes Molekulargewicht: 79 kDa
• GenBank-Zugangsnummer: BC042510
• Gene symbol: CEL
• Gene ID (NCBI): 1056
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Bile salt-activated lipase, previously named cholesterol esterase or bile salt-stimulated (or dependent) lipase, is a 74 kDa lipolytic enzyme capable of hydrolyzing cholesteryl esters, tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide(PMID:12454261). The same enzyme is present as a major protein in milk and as a minor constituent in liver, activated macrophages, and endothelial cells(PMID:11733511). Defects in CEL are a cause of maturity-onset diabetes of the young type 8 with exocrine dysfunction (MODY8)(PMID:16369531). The full length protein has 11 glycosylation sites. It has 2 isoforms produced by alternative splicing.