CENPF Polyklonaler Antikörper

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• KD/KO Validated

CENPF Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 28568-1-AP

Synonyme

PRO1779, CENP-F, CENF

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  WB analysis of HeLa using 28568-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28568-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 28568-1-AP (CENPF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat small intestine using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat small intestine using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat testis using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse small intestine using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skin using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skin tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 28568-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 28568-1-AP (CENPF antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 28568-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 28568-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (28568-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Ratten-Dünndarmgewebe, humanes Lungenkarzinomgewebe, humanes Pankreaskarzinomgewebe, Mausnierengewebe, Mauslebergewebe, Maus-Dünndarmgewebe, Rattenhautgewebe, Rattenhodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

28568-1-AP bindet in WB, IHC, IF/ICC, ELISA CENPF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPF fusion protein Ag29665
• Vollständiger Name: centromere protein F, 350/400ka (mitosin)
• Berechnetes Molekulargewicht: 368 kDa
• Beobachtetes Molekulargewicht: 350 kDa
• GenBank-Zugangsnummer: NM_016343
• Gene symbol: CENPF
• Gene ID (NCBI): 1063
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).

Publikationen

Species	Application	Title
human	WB,IHC	Transl Androl Urol; CENPF promotes the proliferation of renal cell carcinoma in vitro; Authors - Ji Zhang ; KD Validated
human	WB	Nat Commun; CSTF2 mediated mRNA N6-methyladenosine modification drives pancreatic ductal adenocarcinoma m6A subtypes; Authors - Yanfen Zheng
human	IHC,IF	Oncol Lett; FOXM1 and CENPF are associated with a poor prognosis through promoting proliferation and migration in lung adenocarcinoma; Authors - Peipei Li
human	WB	Cancer Cell Int; CENPF interaction with PLA2G4A promotes glioma growth by modulating mTORC1 and NF-κB pathways; Authors - Junhong Li