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CENPF Rekombinanter Antikörper

CENPF Rekombinant Antikörper für IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242277G9

Kat-Nr. : 84865-4-PBS

Synonyme

242277G9, CENF, CENP-F, PRO1779

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPF Recombinant antibody (84865-4-RR)

    IF Staining of HeLa using 84865-4-RR (same clone as 84865-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CENPF Recombinant antibody (84865-4-RR)

    IF Staining of A431 using 84865-4-RR (same clone as 84865-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CENPF antibody (84865-4-RR, Clone: 242277G9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84865-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84865-4-RR

    Affinity and Kinetic Characterization of 84865-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84865-4-RR against Human CENPF were performed. The affinity constant is 11.1 nM.

Geprüfte Anwendungen

Produktinformation

84865-4-PBS bindet in IF/ICC, Indirect ELISA CENPF und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CENPF fusion protein Ag29665
Vollständiger Name centromere protein F, 350/400ka (mitosin)
Berechnetes Molekulargewicht 368 kDa
GenBank-ZugangsnummerNM_016343
Gene symbol CENPF
Gene ID (NCBI) 1063
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CENPF, also named as mitosin and centromere protein F (PMID: 22912832), belongs to centromere protein F family. During the cell cycle, CENPF localizes in multiple cellular structures including the nuclear envelope in late G2/early prophase and kinetochores throughout mitosis (PMID: 30198378). CENPF is required for kinetochore function and chromosome segregation in mitosis and required for kinetochore localization of dynein, LIS1, NDE1 and NDEL1 (PMID: 17600710)(PMID: 7542657). It regulates recycling of the plasma membrane by acting as a link between recycling vesicles and the microtubule network though its association with STX4 and SNAP25. Acts as a potential inhibitor of pocket protein-mediated cellular processes during development by regulating the activity of RB proteins during cell division and proliferation (PMID: 26125848). The calculated molecular weight of CENPF is ~358 kDa and CENPF can be detected as ~367 kDa (PMID: 7542657).