CENPK Polyklonaler Antikörper

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• KD/KO Validated

CENPK Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 26208-1-AP

Synonyme

AF5alpha, CENP K, CENPK, centromere protein K, FKSG14, ICEN37, P33, Protein AF 5alpha, Solt

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  WB analysis of HeLa using 26208-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26208-1-AP (CENPK Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 26208-1-AP

  IP result of anti-CENPK (IP:26208-1-AP, 4ug; Detection:26208-1-AP 1:600) with HeLa cells lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Produktinformation

26208-1-AP bindet in WB, IHC, IP, ELISA CENPK und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPK fusion protein Ag23823
• Vollständiger Name: centromere protein K
• Beobachtetes Molekulargewicht: 32-35 kDa
• GenBank-Zugangsnummer: BC005400
• Gene symbol: CENPK
• Gene ID (NCBI): 64105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Transl Oncol; m6A demethylase ALKBH5 maintains stemness of intrahepatic cholangiocarcinoma by sustaining BUB1B expression and cell proliferation; Authors - Yuan Gao
human	WB,IHC	Cancer Cell Int; Bioinformatics analysis combined with experiments predicts CENPK as a potential prognostic factor for lung adenocarcinoma.; Authors - Jiayu Ma ; KD Validated
human	WB	Toxicol Appl Pharmacol; The inhibition of centromere protein K causes anticancer effects in breast carcinoma via effects on the FAK/PI3K/AKT/mTOR pathway; Authors - Jiao Yu ; KD Validated
human	WB	Bioengineered; Overexpression of centromere protein K (CENPK) gene in Differentiated Thyroid Carcinoma promote cell Proliferation and Migration; Authors - Qizhi Li ; KD Validated
human	WB	Toxicol Appl Pharmacol; Centromere protein K enhances the activation of YAP1/TAZ signal cascade to drive the progression of clear cell renal cell carcinoma; Authors - Ning Nan ; KD Validated
human	WB	Onco Targets Ther; Downregulation of CENPK suppresses hepatocellular carcinoma malignant progression through regulating YAP1.; Authors - Jianlin Wang