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CENPM Polyklonaler Antikörper

CENPM Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19840-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using CENPM Polyclonal antibody (19840-1-AP)

    WB analysis using 19840-1-AP (same clone as 19840-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19840-1-AP (CENPM antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CENPM Polyclonal antibody (19840-1-AP)

    IF Staining of HeLa using 19840-1-AP (same clone as 19840-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using CENPM Polyclonal antibody (19840-1-AP)

    IF Staining of NIH/3T3 using 19840-1-AP (same clone as 19840-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CENPM antibody (19840-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19840-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

19840-1-PBS bindet in WB, IF/ICC, Indirect ELISA CENPM und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CENPM fusion protein Ag13843
Vollständiger Name centromere protein M
Berechnetes Molekulargewicht 180 aa, 20 kDa
Beobachtetes Molekulargewicht20 kDa
GenBank-ZugangsnummerBC000705
Gene symbol CENPM
Gene ID (NCBI) 79019
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Centromere protein M (CENPM), also referred as proliferation associated nuclear element 1 (PANE1), is one of the components of a complex that plays a central role in kinetochore protein assembly, mitotic progression and chromosome segregation. The expression of CENPM and centromere assembly should be controlled closely during the cell cycle, and mistakes in this regulation may result in aneuploidy (PMID: 32705259). CENPM has been reported as a novel biomarker of hepatocellular carcinoma, melanoma and bladder cancer (PMID: 31703591).