CENPO Polyklonaler Antikörper

CENPO Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20611-1-AP

Synonyme

CENP O, CENPO, centromere protein O, ICEN36, MCM21R

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Galerie der Validierungsdaten

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  WB analysis of mouse brain using 20611-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 20611-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 20611-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20611-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20611-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 20611-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, HEK-293-Zellen, HL-60-Zellen, Maus-Eierstockgewebe, Mausnierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

20611-1-AP bindet in WB, ELISA CENPO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPO fusion protein Ag14619
• Vollständiger Name: centromere protein O
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC002870
• Gene symbol: CENPO
• Gene ID (NCBI): 79172
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.