CENPO Polyklonaler Antikörper

CENPO Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20611-1-PBS

Synonyme

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  WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 20611-1-AP (same clone as 20611-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 20611-1-AP (same clone as 20611-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20611-1-AP (same clone as 20611-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 20611-1-AP (same clone as 20611-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse kidney using 20611-1-AP (same clone as 20611-1-PBS)

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20611-1-AP (CENPO antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20611-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20611-1-PBS bindet in WB, Indirect ELISA CENPO und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CENPO fusion protein Ag14619
• Vollständiger Name: centromere protein O
• Berechnetes Molekulargewicht: 300 aa, 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC002870
• Gene symbol: CENPO
• Gene ID (NCBI): 79172
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CENPO is a component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. CENPO may be involved in incorporation of newly synthesized CENPA into centromeres via its interaction with the CENPA-NAC complex. Besides, CENPA modulates the kinetochore-bound levels of NDC80 complex. CENPO has 2 isoforms with MW of 33 and 34 kDa, while different lysates may bind to one or two isforms in SDS-PAGE.