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CEP110 Polyklonaler Antikörper

CEP110 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25235-1-AP

Synonyme

Centriolin, 110 kDa centrosomal protein, Centrosomal protein 1, centrosomal protein 110kDa, Centrosomal protein of 110 kDa

Filter:
  • Western Blot (WB) analysis of Jurkat cells using CEP110 Polyclonal antibody (25235-1-AP)

    WB analysis of Jurkat using 25235-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25235-1-AP (CEP110 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using CEP110 Polyclonal antibody (25235-1-AP)

    IF Staining of MDCK using 25235-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 25235-1-AP (CEP110 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen
Erfolgreiche Detektion in IF/ICCMDCK-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25235-1-AP bindet in WB, IF/ICC, ELISA CEP110 und zeigt Reaktivität mit human, Hund

Getestete Reaktivität human, Hund
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CEP110 fusion protein Ag18907
Vollständiger Name centrosomal protein 110kDa
Berechnetes Molekulargewicht 2325 aa, 269 kDa
Beobachtetes Molekulargewicht270 kDa
GenBank-ZugangsnummerBC137284
Gene symbol CEP110
Gene ID (NCBI) 11064
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CEP110 antibody 25235-1-APProtokoll herunterladen
IF protocol for CEP110 antibody 25235-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Jay (Verified Customer) (10-28-2019)

Methanol fixation.The first IF result showing that cep110 localizes at centrosome in mouse NIH3T3 cell line(Red;Cep110, Green;gamma-Tubulin for centrosome marker, Blue;DAPI for nucleus.)

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse NIH3T3 cell line
CEP110 Antibody Immunofluorescence, validation (1:100 dilution) in Mouse NIH3T3 cell line (Cat no:25235-1-AP)