CEP152 Polyklonaler Antikörper

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CEP152 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21815-1-PBS

Synonyme

KIAA0912, centrosomal protein 152kDa, CEP 152

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  WB analysis using 21815-1-AP (same clone as 21815-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21815-1-AP (CEP152 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 21815-1-AP (same clone as 21815-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21815-1-AP (CEP152 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 21815-1-AP (same clone as 21815-1-PBS)

  IP result of anti-CEP152 (IP:21815-1-AP, 4ug; Detection:21815-1-AP 1:2000) with mouse brain tissue lysate 1280 ug. This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21815-1-AP (same clone as 21815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21815-1-AP (CEP152 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 21815-1-AP (same clone as 21815-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 21815-1-AP (CEP152 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21815-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21815-1-PBS bindet in WB, IHC, Indirect ELISA CEP152 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: centrosomal protein 152kDa
• Berechnetes Molekulargewicht: 189 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: NM_014985
• Gene symbol: CEP152
• Gene ID (NCBI): 22995
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP152, also named as KIAA0912, is a regulator of genomic integrity and cellular response to DNA damage acting through ATR-mediated checkpoint signaling. It is necessary for centrosome duplication. CEP152 functions as a molecular scaffold facilitating the interaction of PLK4 and CENPJ, two molecules involved in centriole formation. CEP152, ATR and CENPJ control cellular responses to DNA damage. CEP152 has some isoforms with MW 189 kDa, 152 kDa, 110 kDa and 66 kDa.