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CEP164 Polyklonaler Antikörper

CEP164 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(74)

Kat-Nr. : 22227-1-AP

Synonyme

3F2B7, CEP 164, KIAA1052, centrosomal protein 164kDa, NPHP15



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen
Erfolgreiche IPRPE1-Zellen
Erfolgreiche Detektion in IHChumanes Kolongewebe, humanes Zervixkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHeLa-Zellen, A549-Zellen, hTERT-RPE1-Zellen, MDCK-Zellen, PC-3-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:200-1:1000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:100-1:1000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

22227-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CEP164 und zeigt Reaktivität mit human, Hund

Getestete Reaktivität human, Hund
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CEP164 fusion protein Ag17570
Vollständiger Name centrosomal protein 164kDa
Berechnetes Molekulargewicht 1460 aa, 164 kDa
Beobachtetes Molekulargewicht 164 kDa
GenBank-ZugangsnummerBC000602
Gene symbol CEP164
Gene ID (NCBI) 22897
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP164, also called KIAA1052 or NPHP15, is a 1460 amino acid protein containing 1 WW domain. CEP164 localizes in the microtubule organizing center and is expressed in several cell lines. CEP164 plays a role in microtubule organization and/or maintenance for the formation of primary cilia, a microtubule-based structure that protrudes from the surface of epithelial cells. CEP164 plays a critical role in the G2/M checkpoint and nuclear divisions. The expression of CEP164 is normally limited to the mother centriole, and CEP164 can be used as a useful marker for mother centriole.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CEP164 antibody 22227-1-APProtokoll herunterladen
IHC protocol for CEP164 antibody 22227-1-APProtokoll herunterladenl
IF protocol for CEP164 antibody 22227-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseIF

Cell Res

NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.

Authors - Min Liu
humanIF

Nat Commun

M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.

Authors - Ning Huang

Nat Commun

Sub-centrosomal mapping identifies augmin-γTuRC as part of a centriole-stabilizing scaffold.

Authors - Nina Schweizer

Nat Commun

Genetic link between renal birth defects and congenital heart disease.

Authors - Jovenal T San Agustin
humanWB

Nat Commun

DNA replication licensing factor Cdc6 and Plk4 kinase antagonistically regulate centrosome duplication via Sas-6.

Authors - Xiaowei Xu
humanIF

EMBO J

ANKRD26 recruits PIDD1 to centriolar distal appendages to activate the PIDDosome following centrosome amplification.

Authors - Lauren T Evans

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Ludovic (Verified Customer) (02-27-2024)

A fabulous antibody to identify the distal appendage of the primary cilium. Never seen an antibody that works so well!

  • Applications: Immunofluorescence, Cell culture
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human cell line
FH

Elisa (Verified Customer) (04-04-2023)

H9 cells stained for Hoechst (DNA marker, in green), Cep164 (mother centriole distal appendage marker, in green) and g-Tubulin (pericentriolar matrix marker, in magenta). H9 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:300 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:300
  • Cell Tissue Type: H9 cells
CEP164 Antibody Immunofluorescence validation (1:300 dilution) in H9 cells (Cat no:22227-1-AP)
FH

Pierrick (Verified Customer) (10-24-2019)

Great antibody for immunofluorescence on human cells.Works well in WB

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: CEP164 IF 1/1000 to 1/2000 and WB 1/1000
  • Cell Tissue Type: RPE1, HEK290, HeLa Kyoto