CEP250,C-NAP1 Monoklonaler Antikörper

CEP250,C-NAP1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

CloneNo.

2G12D12

Kat-Nr. : 66814-1-Ig

Synonyme

Cep250, CEP250/CNAP1, 250 kDa centrosomal protein, 2G12D12, C NAP1

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  WB analysis using 66814-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 66814-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 2 publications below

Produktinformation

66814-1-Ig bindet in WB, IHC, IF/ICC, ELISA CEP250,C-NAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CEP250,C-NAP1 fusion protein Ag5925
• Vollständiger Name: centrosomal protein 250kDa
• Berechnetes Molekulargewicht: 281 kDa
• Beobachtetes Molekulargewicht: 280 kDa
• GenBank-Zugangsnummer: BC001433
• Gene symbol: CEP250/CNAP1
• Gene ID (NCBI): 11190
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.

Publikationen

Species	Application	Title
mouse,human	IHC,IF	iScience; Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components; Authors - Luigi Cerulo
human	IHC,IF	Pancreatology; Altered centriolar cohesion by CEP250 and appendages impact outcome of patients with pancreatic cancer; Authors - Guido Giordano