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CEP250,C-NAP1 Monoklonaler Antikörper

CEP250,C-NAP1 Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2G12D12

Kat-Nr. : 66814-1-PBS

Synonyme

Cep250, CEP250/CNAP1, 250 kDa centrosomal protein, 2G12D12, C NAP1

Filter:
  • Western Blot (WB) analysis of various lysates using CEP250,C-NAP1 Monoclonal antibody (66814-1-Ig)

    WB analysis using 66814-1-Ig (same clone as 66814-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66814-1-Ig (CEP250,C-NAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CEP250,C-NAP1 Monoclonal antibody (66814-1-Ig)

    IF Staining of HepG2 using 66814-1-Ig (same clone as 66814-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CEP250,C-NAP1 antibody (66814-1-Ig, Clone: 2G12D12 ) at dilution of 1:773 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66814-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66814-1-PBS bindet in WB, IF/ICC, ELISA CEP250,C-NAP1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CEP250,C-NAP1 fusion protein Ag5925
Vollständiger Name centrosomal protein 250kDa
Berechnetes Molekulargewicht 281 kDa
Beobachtetes Molekulargewicht 280 kDa
GenBank-ZugangsnummerBC001433
Gene symbol CEP250/CNAP1
Gene ID (NCBI) 11190
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP250, also known as C-Nap1, is a coiled-coil protein that localizes to the proximal ends of mother and daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome.