CEP290 Polyklonaler Antikörper

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• KD/KO Validated

CEP290 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22490-1-PBS

Synonyme

CT87, Centrosomal protein of 290 kDa, Cancer/testis antigen 87, BBS14, Bardet-Biedl syndrome 14 protein

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  WB analysis of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22490-1-AP (CEP290 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

  IP result of anti-CEP290 (IP:22490-1-AP, 4ug; Detection:22490-1-AP 1:3000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22490-1-AP (same clone as 22490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 22490-1-AP (same clone as 22490-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22490-1-AP (CEP290 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 22490-1-AP (same clone as 22490-1-PBS)

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 22490-1-AP (CEP290 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 22490-1-AP (same clone as 22490-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CEP290 antibody (22490-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 22490-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

22490-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CEP290 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP290 fusion protein Ag17457
• Vollständiger Name: centrosomal protein 290kDa
• Berechnetes Molekulargewicht: 2479 aa, 290 kDa
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: BC008641
• Gene symbol: CEP290
• Gene ID (NCBI): 80184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt. Catalog#22490-1-AP can recognise both.