CEP290 Monoklonaler Antikörper

CEP290 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2C10G2

Kat-Nr. : 67716-1-PBS

Synonyme

3H11Ag, BBS14, Cancer/testis antigen 87, centrosomal protein 290kDa, Centrosomal protein of 290 kDa, CEP290, CT87, FLJ13615, FLJ21979, JBTS5, JBTS6, KIAA0373, LCA10, MKS4, Nephrocystin 6, rd16, SLSN6, Tumor antigen se2 2

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  WB analysis using 67716-1-Ig (same clone as 67716-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67716-1-Ig (CEP290 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67716-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67716-1-Ig (same clone as 67716-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67716-1-Ig (CEP290 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67716-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67716-1-PBS bindet in WB, Indirect ELISA CEP290 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CEP290 fusion protein Ag17457
• Vollständiger Name: centrosomal protein 290kDa
• Berechnetes Molekulargewicht: 2479 aa, 290 kDa
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: BC008641
• Gene symbol: CEP290
• Gene ID (NCBI): 80184
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Studies in the Chlamydomonas model system has shown that the ciliary protein CEP290 is a critical component of these Y-link junctions. Additionally, numerous studies have demonstrated that CEP290's function is critical for IFT-in CEP290 knockdown experiments, many proteins that would normally localize to the cilium fail to do so, and cilium formation is disrupted or absent. Mutations in CEP290 are accountable for cases of nephronophthisis, Leber congenital amaurosis and Joubert syndrome. In IF analtsis of HeLa cells, the white arrows show centrosome and cilium staining. Two isoforms were produced by alternative splicing with predicted MW of 290 and 180 kDa according to UniProt.