CEP350-Specific Polyklonaler Antikörper

CEP350-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20022-1-PBS

Synonyme

Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa

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  WB analysis using 20022-1-AP (same clone as 20022-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20022-1-AP (CEP350-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20022-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 20022-1-AP (same clone as 20022-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20022-1-AP (CEP350-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20022-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 20022-1-AP (same clone as 20022-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CEP350-Specific antibody (20022-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 20022-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20022-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CEP350-Specific und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: centrosomal protein 350kDa
• Berechnetes Molekulargewicht: 351 kDa
• Beobachtetes Molekulargewicht: 245 kDa
• GenBank-Zugangsnummer: NM_014810
• Gene symbol: CEP350
• Gene ID (NCBI): 9857
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP350, or Centrosomal Protein 350 kDa, also named CAP350, FLJ38282, FLJ44058, GM133, and KIAA0480, is a protein that plays a crucial role in the formation and maintenance of the centrosome, which is essential for cell division. The molecular weight of CEP350 varies depending on the isoforms and post-translational modifications. the molecular weight of CEP350 can range from 200-245 kDa, 143 kDa, to 65 kDa. These variations in molecular weight are likely due to different isoforms of the protein that arise from alternative splicing or other post-translational modifications.