CEP44 Polyklonaler Antikörper

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• KD/KO Validated

CEP44 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24457-1-PBS

Synonyme

Centrosomal protein of 44 kDa, HBV PreS1-transactivated protein 3, PS1TP3

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  WB analysis using 24457-1-AP (same clone as 24457-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse testis using 24457-1-AP (same clone as 24457-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24457-1-AP (CEP44 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 24457-1-AP (same clone as 24457-1-PBS)

  IP result of anti-CEP44 (IP:24457-1-AP, 4ug; Detection:24457-1-AP 1:3000) with HeLa cells lysate 1640 ug. This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24457-1-AP (CEP44 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 24457-1-AP (same clone as 24457-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain slide using 24457-1-AP (CEP44 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 24457-1-AP (same clone as 24457-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MDCK cells using CEP44 antibody (24457-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 24457-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

24457-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CEP44 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP44 fusion protein Ag19961
• Vollständiger Name: KIAA1712
• Berechnetes Molekulargewicht: 390 aa, 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC038667
• Gene symbol: CEP44
• Gene ID (NCBI): 80817
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP44, also named as KIAA1712 and PS1TP3, localizes to centrosomes, spindle poles, and weakly in midbody. It is a 44 kDa centrosome protein.