CEP72 Polyklonaler Antikörper

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• KD/KO Validated

CEP72 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 19928-1-AP

Synonyme

KIAA1519, Centrosomal protein of 72 kDa, centrosomal protein 72kDa

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  WB analysis of HEK-293 using 19928-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 19928-1-AP (CEP72 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 19928-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19928-1-AP (CEP72 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 19928-1-AP

  IP result of anti-CEP72 (IP:19928-1-AP, 4ug; Detection:19928-1-AP 1:1000) with HeLa cells lysate 520ug.

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  IF Staining of HeLa using 19928-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP72 antibody (19928-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

• 

  IF Staining of HeLa using 19928-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CEP72 antibody (19928-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, HEK-293-Zellen, Rattenhodengewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

19928-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CEP72 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP72 fusion protein Ag13905
• Vollständiger Name: centrosomal protein 72kDa
• Berechnetes Molekulargewicht: 646 aa, 72 kDa
• Beobachtetes Molekulargewicht: 72 kDa
• GenBank-Zugangsnummer: BC001750
• Gene symbol: CEP72
• Gene ID (NCBI): 55722
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB, IP, IF	Elife; Centriolar satellites assemble centrosomal microcephaly proteins to recruit CDK2 and promote centriole duplication.; Authors - Andrew Kodani ; KD Validated
human	IF	EMBO Rep; Trichoplein binds PCM1 and controls endothelial cell function by regulating autophagy.; Authors - Andrea Martello
human	IF	J Cell Sci; Centriolar satellite protein CCDC66 interacts with CEP290 and functions in cilium formation and trafficking.; Authors - Deniz Conkar
human	WB	Sci Rep; Centrosomal and ciliary targeting of CCDC66 requires cooperative action of centriolar satellites, microtubules and molecular motors.; Authors - Deniz Conkar ; KD Validated
human	IF	J Biol Chem; Polo-like kinase 4 maintains centriolar satellite integrity by phosphorylation of centrosomal protein 131 (CEP131).; Authors - Ryan A Denu
human	WB,IF	BMC Biol; CCHCR1-astrin interaction promotes centriole duplication through recruitment of CEP72; Authors - Zhenguang Ying