CEP83 Polyklonaler Antikörper

CEP83 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26013-1-PBS

Synonyme

---

• 

  WB analysis of HEK-293 using 26013-1-AP (same clone as 26013-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 26013-1-AP (CEP83 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 26013-1-AP (same clone as 26013-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26013-1-AP (CCDC41 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 ARL13B antibody (CL594-17711, red). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

• 

  IF Staining of hTERT-RPE1 using 26013-1-AP (same clone as 26013-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP83 antibody (26013-1-AP) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 26013-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

26013-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CEP83 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP83 fusion protein Ag22826
• Vollständiger Name: coiled-coil domain containing 41
• Berechnetes Molekulargewicht: 693 aa, 82 kDa
• Beobachtetes Molekulargewicht: 83 kDa, 68 kDa
• GenBank-Zugangsnummer: BC125087
• Gene symbol: CEP83
• Gene ID (NCBI): 51134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP83 (centrosomal protein, 83 kDa), also known as CCDC41 (coiled-coil domain containing 41), is required for ciliary vesicle docking to the mother centriole. It is a key component of the centriolar distal appendages. CCDC41 may collaborate with IFT20 in the trafficking of ciliary membrane proteins from the Golgi complex to the cilium during the initiation of primary cilium assembly (PMID: 23530209). Alternative splicing results in transcript variants encoding two isoforms with calculated molecular weights of 83 kDa and 68 kDa, respectively.