CEP89, CCDC123 Polyklonaler Antikörper

CEP89, CCDC123 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30165-1-AP

Synonyme

CEP89, CCDC123, Coiled-coil domain-containing protein 123, Cep123, Centrosomal protein of 89 kDa

---

• 

  WB analysis of A549 using 30165-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 30165-1-AP (CCDC123 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of hTERT-RPE1 using 30165-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CCDC123 antibody (30165-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30165-1-AP bindet in WB, IF/ICC, ELISA CEP89, CCDC123 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP89, CCDC123 fusion protein Ag32789
• Vollständiger Name: coiled-coil domain containing 123
• Berechnetes Molekulargewicht: 783 aa, 90 kDa
• Beobachtetes Molekulargewicht: 85-100 kDa
• GenBank-Zugangsnummer: BC136328
• Gene symbol: CEP89
• Gene ID (NCBI): 84902
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CCDC123(as known as CEP123), also named as CEP89, is a new player in the process of primary ciliogenesis and it also plays a role in mitochondrial metabolism where it may modulate complex IV activity. It has been shown that CEP123 is localized to the distal appendages of the mother centriolecep and the localization of CEP123 is cell cycle-dependent with its levels decreasing during mitosis. CEP123 depletion can cause defects in ciliary vesicle formationcep and prevent the formation of a ciliary vesicle at the distal end of the mother centriole. It is possible that CEP123 is involved in regulating the recruitment of membranes to the centrosome through its interaction with Cep290(PMID:23575228, 23789104, 23348840). 24002-1-AP antibody recognizes all of CEP123 isoforms.