CEP97 Polyklonaler Antikörper

CEP97 Polyklonal Antikörper für IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund und mehr (2)

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 22050-1-AP

Synonyme

2810403B08Rik, centrosomal protein 97kDa, Centrosomal protein of 97 kDa, CEP97, LRRIQ2

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Galerie der Validierungsdaten

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  WB analysis of HL-60 using 22050-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 22050-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 22050-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 22050-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 22050-1-AP

  IP Result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug.

• 

  IHC staining of human breast cancer using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 22050-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of MDCK using 22050-1-AP

  Immunofluorescent analysis of MDCK cells using 22050-1-AP (CEP97 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HEK-293-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MDCK-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 11 publications below
• IF: See 12 publications below
• IP: See 2 publications below

Produktinformation

22050-1-AP bindet in WB, IP, IHC, IF, ELISA CEP97 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• In Publikationen genannte Reaktivität: human, drosophila, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP97 fusion protein Ag16995
• Vollständiger Name: centrosomal protein 97kDa
• Berechnetes Molekulargewicht: 865 aa, 97 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC041085
• Gene symbol: CEP97
• Gene ID (NCBI): 79598
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.

Publikationen

Species	Application	Title
mouse	WB	Cell Res; NudCL2 is an autophagy receptor that mediates selective autophagic degradation of CP110 at mother centrioles to promote ciliogenesis.; Authors - Min Liu
human,mouse	WB,IF	Nat Commun; M-Phase Phosphoprotein 9 regulates ciliogenesis by modulating CP110-CEP97 complex localization at the mother centriole.; Authors - Ning Huang
human	WB	J Cell Biol; Centrin2 regulates CP110 removal in primary cilium formation.; Authors - Suzanna L Prosser
Drosophila	IF,WB	Curr Biol; Cep97 Is Required for Centriole Structural Integrity and Cilia Formation in Drosophila.; Authors - Jeroen Dobbelaere
mouse	IF	Curr Biol; Mother Centriole Distal Appendages Mediate Centrosome Docking at the Immunological Synapse and Reveal Mechanistic Parallels with Ciliogenesis.; Authors - Jane C Stinchcombe
human	WB,IF	Am J Hum Genet; Truncated SALL1 Impedes Primary Cilia Function in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra