CEP97 Polyklonaler Antikörper

CEP97 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Hund

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 22050-1-PBS

Synonyme

---

• 

  WB analysis of HL-60 using 22050-1-AP (same clone as 22050-1-PBS)

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 22050-1-AP (same clone as 22050-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 22050-1-AP (same clone as 22050-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22050-1-AP (CEP97 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 22050-1-AP (same clone as 22050-1-PBS)

  IP result of anti-CEP97 (IP:22050-1-AP, 4ug; Detection:22050-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 22050-1-AP (same clone as 22050-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver using 22050-1-AP (CEP97 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MDCK using 22050-1-AP (same clone as 22050-1-PBS)

  Immunofluorescent analysis of MDCK cells using 22050-1-AP (CEP97 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 22050-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

22050-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA CEP97 und zeigt Reaktivität mit human, Hund

• Getestete Reaktivität: human, Hund
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CEP97 fusion protein Ag16995
• Vollständiger Name: centrosomal protein 97kDa
• Berechnetes Molekulargewicht: 865 aa, 97 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC041085
• Gene symbol: CEP97
• Gene ID (NCBI): 79598
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CEP97, also named as LRRIQ2, contains one IQ domain, eight LRR (leucine-rich) repeats and one LRRCT domain. It collaborates with CEP110, being involved in the suppression of a cilia assembly program. CEP97 is required for correct spindle formation and has a role in cytokinesis. It is required for recruitment of CEP110 to the centrosome. This protein has a calculated MW ~97 kDa, although the protein migrates at a molecular weight of approximate 140 kDa. The protein is highly acidic, with a pI ~4.7, providing a potential explanation for its reduced mobility in SDS-PAGE. [PMID: 17719545]. This is a rabbit polyclonal antibody raised against the C terminus of human CEP97.