Centrin 1 Monoklonaler Antikörper

Centrin 1 Monoklonal Antikörper für IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

1G8D10

Kat-Nr. : 68433-1-Ig

Synonyme

Caltractin isoform 2, CEN1, Centrin 1, centrin, EF hand protein, 1, CETN, CETN1

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  IF Staining of hTERT-RPE1 using 68433-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of ARPE-19 using 68433-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed ARPE-19 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of hTERT-RPE1 using 68433-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CETN1 antibody (68433-1-Ig, Clone: 1G8D10 ) at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen, ARPE-19 cells

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

68433-1-Ig bindet in IF/ICC, ELISA Centrin 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Centrin 1 fusion protein Ag3529
• Vollständiger Name: centrin, EF-hand protein, 1
• Berechnetes Molekulargewicht: 172 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC029515
• Gene symbol: Centrin 1
• Gene ID (NCBI): 1068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Centrin-1 plays important roles in the determination of centrosome position and segregation, and in the process of microtubule severing. Centrin-1 is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle.

Publikationen

Species	Application	Title
human	IF	Oncogene; LIMK2 promotes centrosome clustering and cancer progression by activating MST4-mediated phosphorylation of NPM1; Authors - Jie Tian