Centrin 3 Polyklonaler Antikörper

Centrin 3 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 15811-1-AP

Synonyme

CETN3, CEN3, Centrin3, Centrin-3, CETN 3

---

• 

  WB analysis using 15811-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15811-1-AP (Centrin 3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse kidney using 15811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15811-1-AP (Centrin 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 15811-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15811-1-AP (Centrin 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 15811-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Centrin 3 antibody (15811-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NCI-H1299-Zellen, C2C12-Zellen, Maus-Eierstockgewebe, Maushodengewebe, Neuro-2a-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:6000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

15811-1-AP bindet in WB, IHC, IF/ICC, ELISA Centrin 3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Centrin 3 fusion protein Ag8547
• Vollständiger Name: centrin, EF-hand protein, 3 (CDC31 homolog, yeast)
• Berechnetes Molekulargewicht: 167 aa, 20 kDa
• Beobachtetes Molekulargewicht: 23 kDa
• GenBank-Zugangsnummer: BC005383
• Gene symbol: Centrin 3
• Gene ID (NCBI): 1070
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Centrin 3 protein is a highly conserved calcium-binding protein that belongs to the EF-hand calcium-binding protein superfamily and contains four calcium-binding domains. It is primarily located in structures associated with the microtubule-organizing center within cells. Centrin 3 plays a crucial role in centriole duplication, which is essential for the bipolarity of cell division. Additionally, Centrin 3 is involved in inhibiting the activity of the centrosomal Mps1 kinase and has an antagonistic relationship with the function of Centrin 2. Recent studies have shown that CETN3 interacts with USP49 to participate in RNA splicing processes in neural stem/progenitor cells, thereby indirectly affecting the cell cycle and neural development.

Publikationen

Species	Application	Title
human	WB	EMBO Rep; Zika virus alters centrosome organization to suppress the innate immune response.; Authors - Andrew Kodani