CFAP47 Polyklonaler Antikörper

CFAP47 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30579-1-AP

Synonyme

CXorf59, Cilia and flagella associated protein 47

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  WB analysis using 30579-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30579-1-AP (CFAP47 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 30579-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 30579-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30579-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 30579-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 30579-1-AP (CFAP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 30579-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CFAP47 antibody (30579-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MRC-5 cells, humanes Hodengewebe, Maus-Spermatophoren-Gewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

30579-1-AP bindet in WB, IHC, IF/ICC, ELISA CFAP47 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CFAP47 fusion protein Ag31679
• Vollständiger Name: chromosome X open reading frame 59
• Beobachtetes Molekulargewicht: 100-110 kDa
• GenBank-Zugangsnummer: BC101700
• Gene symbol: CXorf59
• Gene ID (NCBI): 286464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CFAP47 (Cilia- and flagella-associated protein 47), formerly known as CXorf22, CXorf59, is located on the human chromosome X and highly expressed in the testis and encodes a cilia- and flagella-associated protein. CFAP47 was expressed in primary cilia of human kidney tubules, and knockout (KO) mice exhibited vacuolation of tubular cells and tubular dilation, providing evidence that CFAP47 is a causative gene involved in cyst formation (PMID: 33472045, 38633811). CFAP47-mutated male mice are sterile, with decreased sperm motility and abnormal flagella morphology (PMID: 37424856).