CFAP70 Polyklonaler Antikörper

CFAP70 Polyklonal Antikörper für IHC, IF-P, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IHC, IF-P, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31344-1-PBS

Synonyme

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  IP experiment of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

  IP result of anti-CFAP70 (IP:31344-1-AP, 4ug; Detection:31344-1-AP 1:300) with mouse testis tissue lysate 1120 ug. This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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  IHC staining of rat testis using 31344-1-AP (same clone as 31344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31344-1-AP (CFAP70 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse testis using 31344-1-AP (same clone as 31344-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CFAP70 antibody (31344-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31344-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31344-1-PBS bindet in IHC, IF-P, IP, Indirect ELISA CFAP70 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CFAP70 fusion protein Ag35209
• Vollständiger Name: tetratricopeptide repeat domain 18
• Berechnetes Molekulargewicht: 126 kDa
• Beobachtetes Molekulargewicht: 126 kDa
• GenBank-Zugangsnummer: BC032856
• Gene symbol: TTC18
• Gene ID (NCBI): 118491
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cilia- and flagella-associated protein 70 (CFAP70) harbours eight tetratricopeptide repeats (TPRs) and bound tightly to the ciliary axoneme. CFAP70 is necessary to assemble spermatid flagella and could be used as a diagnostic target for male infertility with OAT in the clinic.CFAP70 is a novel regulatory component of the ODA in motile cilia and flagella, and that the N-terminus is important for its ciliary localization (PMID: 30158508).