Complement factor B Polyklonaler Antikörper

Complement factor B Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Rezensionen (1)

Kat-Nr. : 10170-1-AP

Synonyme

BF, BFD, C3/C5 convertase, CFAB, CFB, Complement factor B, FB, FBI12, GBG, Glycine rich beta glycoprotein, H2 Bf, PBF2, Properdin factor B

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Galerie der Validierungsdaten

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  WB analysis using 10170-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human plasma using 10170-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human blood using 10170-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 10170-1-AP (Complement factor B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 10170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10170-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10170-1-AP (Complement factor B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, human blood, rat plasma
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

10170-1-AP bindet in WB, IHC, IF, ELISA Complement factor B und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Complement factor B fusion protein Ag0226
• Vollständiger Name: complement factor B
• Berechnetes Molekulargewicht: 86 kDa
• Beobachtetes Molekulargewicht: 93-100 kDa, 60-69 kDa
• GenBank-Zugangsnummer: BC007990
• Gene symbol: CFB
• Gene ID (NCBI): 629
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Complement factor B (CFB) is a component of the alternative pathway of complement activation. Complement factor B is a 93-100 kDa glycoprotein and is cleaved into Ba (33 kDa) and Bb (60 kDa) by factor D in the presence of C3b (PMID: 11025450). The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This antibody raised against 573-761aa of human complement factor B can recognize complement factor B and Bb fragment.

Publikationen

Species	Application	Title
human,mouse	IF	Nat Commun; The classical pathway triggers pathogenic complement activation in membranous nephropathy; Authors - Larissa Seifert
human	WB	J Cell Physiol; IL-1/IL-1R signaling induced by all-trans-retinal contributes to complement alternative pathway activation in retinal pigment epithelium.; Authors - Xinxuan Cheng
human	WB,IHC,IF	Cell Oncol (Dordr); Complement factor B regulates cellular senescence and is associated with poor prognosis in pancreatic cancer.; Authors - Reiri Shimazaki
human	WB	Br J Cancer; Identification of GlcNAcylated alpha-1-antichymotrypsin as an early biomarker in human non-small-cell lung cancer by quantitative proteomic analysis with two lectins.; Authors - Yanxia Jin
human,mouse	WB	Nephrol Dial Transplant; Resveratrol delays polycystic kidney disease progression through attenuation of nuclear factor κB-induced inflammation.; Authors - Ming Wu
	WB	Chem Res Toxicol; 4-Hydroxy-7-oxo-5-heptenoic Acid Lactone Is a Potent Inducer of the Complement Pathway in Human Retinal Pigmented Epithelial Cells.; Authors - Mikhail Linetsky

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

mark (Verified Customer) (03-30-2020)

worked with colourmetric immunohistochemistry

• Applications: Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: E13.5 to E18.5 embryo

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