CFHR3 Polyklonaler Antikörper

CFHR3 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 16583-1-AP

Synonyme

CFHL3, CFHR3, complement factor H related 3, DOWN16, FHR 3, FHR3, H factor like protein 3, HLF4

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Galerie der Validierungsdaten

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  WB analysis using 16583-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 16583-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 16583-1-AP (CFHR3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16583-1-AP

  IP Result of anti-CFHR3 (IP:16583-1-AP, 4ug; Detection:16583-1-AP 1:300) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver using 16583-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 16583-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16583-1-AP (CFHR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, humanes Herzgewebe, Rattenlebergewebe
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

16583-1-AP bindet in WB, IP, IHC, IF, ELISA CFHR3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CFHR3 fusion protein Ag9963
• Vollständiger Name: complement factor H-related 3
• Berechnetes Molekulargewicht: 330 aa, 37 kDa
• Beobachtetes Molekulargewicht: 45-56 kDa
• GenBank-Zugangsnummer: BC058009
• Gene symbol: CFHR3
• Gene ID (NCBI): 10878
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CFHR3, also named as DOWN16, belongs to the complement factor H-related protein family. Expressed by the liver and secreted in plasma, human CFHR3 is composed of five short consensus repeats (SCRs), and it also has a 19-amino acid signal peptide and four N-linked glycosylation sites. It may be involved in complement regulation. Deletion of CFHR1 and CFHR3 genes was found to be associated with decreased risk of age-related macular degeneration (AMD), and with an increased risk of atypical hemolytic-uremic syndrome (aHUS).

Publikationen

Species	Application	Title
human	WB	Clin Cancer Res; Complement regulatory proteins CFHR1 and CFHR3 and patient response to anti-CD20 monoclonal antibody therapy.; Authors - Laura M Rogers
human	WB	Sci Rep; A haplotype in CFH family genes confers high risk of rare glomerular nephropathies.; Authors - Yin Ding
human	IF	Exp Eye Res; Quantitative analysis of hydroxyapatite-binding plasma proteins in genotyped individuals with late-stage age-related macular degeneration.; Authors - Swati Arya
human	WB	PLoS One; A novel quantitative hemolytic assay coupled with restriction fragment length polymorphisms analysis enabled early diagnosis of atypical hemolytic uremic syndrome and identified unique predisposing mutations in Japan.; Authors - Yoko Yoshida
human	WB,IHC	Bioengineered; A novel hypoxia-driven gene signature that can predict the prognosis of hepatocellular carcinoma.; Authors - Zhirui Zeng
human	WB	Mol Med Rep; Complement factor H‑related 3 overexpression affects hepatocellular carcinoma proliferation and apoptosis.; Authors - Hong Liu

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