CFTR Monoklonaler Antikörper

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• KD/KO Validated

CFTR Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2F4A8

Kat-Nr. : 66928-1-PBS

Synonyme

Channel conductance-controlling ATPase, CFTR/MRP, cAMP-dependent chloride channel, ATP-binding cassette sub-family C member 7, ABCC7

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  WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66928-1-Ig (CFTR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  WB analysis using 66928-1-Ig (same clone as 66928-1-PBS)

  WB result of CFTR antibody (66928-1-Ig; 1:4000; room temperature for 1.5 hours) with wild-type and CFTR knockout HEK-293T cells. This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung using 66928-1-Ig (same clone as 66928-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 66928-1-Ig (CFTR antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66928-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66928-1-PBS bindet in WB, IHC, ELISA CFTR und zeigt Reaktivität mit human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CFTR fusion protein Ag27810
• Vollständiger Name: cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)
• Berechnetes Molekulargewicht: 168 kDa
• Beobachtetes Molekulargewicht: 150 kDa
• GenBank-Zugangsnummer: NM_000492
• Gene symbol: CFTR
• Gene ID (NCBI): 1080
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CFTR is a member of the ATP-binding cassette (ABC) family of membrane transport proteins, functioning as a chloride channel responsible for ion flow across epithelial surfaces of lung, sinuses, pancreas, intestine, and liver. Mutations of CFTR cause cystic fibrosis (CF), a disorder affecting the respiratory, digestive, reproductive systems and sweat glands.