Cingulin Polyklonaler Antikörper

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• KD/KO Validated

Cingulin Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 21369-1-AP

Synonyme

KIAA1319, CGN

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  WB analysis using 21369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21369-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21369-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 21369-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 21369-1-AP

  IP result of anti-Cingulin (IP:21369-1-AP, 4ug; Detection:21369-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human ovary cancer using 21369-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21369-1-AP (Cingulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, MCF-7-Zellen, Mausnierengewebe, Maus-Dünndarmgewebe
• Erfolgreiche IP: Mausnierengewebe
• Erfolgreiche Detektion in IHC: human ovary cancer tissue; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

21369-1-AP bindet in WB, IHC, IF, IP, ELISA Cingulin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hund, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Cingulin fusion protein Ag15843
• Vollständiger Name: cingulin
• Berechnetes Molekulargewicht: 1197 aa, 136 kDa
• Beobachtetes Molekulargewicht: 136-150 kDa
• GenBank-Zugangsnummer: BC146657
• Gene symbol: Cingulin
• Gene ID (NCBI): 57530
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.

Publikationen

Species	Application	Title
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
canine	WB,IF	Nat Commun; Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.; Authors - Anthony J Mangan ; KO Validated
mouse	WB,IF	Mucosal Immunol; Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.; Authors - Jiayao Ou
human	WB,IHC	Cancers (Basel); miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.; Authors - Xiaohui Zhang
rat	WB	Sci Rep; Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats; Authors - Masashi Mukohda
mouse	WB, IP	Cell Signal; Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate.; Authors - Serge Ducommun