CHAC1 Polyklonaler Antikörper

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• KD/KO Validated

CHAC1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human, Maus, Ratte und mehr (2)

Anwendung

WB, IHC, IF, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(38)

Rezensionen (1)

Kat-Nr. : 15207-1-AP

Synonyme

Blocks Notch protein, Botch, Cation transport regulator-like protein 1, EC:4.3.2.7, Gamma-GCG 1

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  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15207-1-AP (CHAC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15207-1-AP

  WB result of CHAC1 antibody (15207-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHAC1 transfected HeLa cells.

• 

  IHC staining of human stomach using 15207-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 15207-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 15207-1-AP (CHAC1 antibody) at dilution of 1:200 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, C6-Zellen, CHO-Zellen, RAW 264.7-Zellen, U-251-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 33 publications below
• IHC: See 10 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15207-1-AP bindet in WB, IHC, IF, IP, CoIP, ELISA CHAC1 und zeigt Reaktivität mit hamster, human, Maus, Ratten

• Getestete Reaktivität: hamster, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hefe, Huhn, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHAC1 fusion protein Ag7360
• Vollständiger Name: ChaC, cation transport regulator homolog 1 (E. coli)
• Berechnetes Molekulargewicht: 24 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC001847
• Gene symbol: CHAC1
• Gene ID (NCBI): 79094
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHAC1(Cation transport regulator-like protein 1) was identified in a co-regulated group of genes enriched for components of the ATF4 (activating transcription factor 4) arm of the unfolded protein response pathway. CHAC1 is a proapoptotic ER stress protein downstream of the pancreatic EIF2α kinase-ATF4 pathway that appears to be important for human physiology and disease(PMID: 19109178). In addition, the study found that high CHAC1 expression is associated with a bad prognosis hinting that CHAC1 may have a possible prognostic significance in breast cancer(PMID: 35930144). The predicted molecular weight of CHAC1 is 24 kDa, and the molecular weight detected by Proteintech is 38 kDa (PMID: 39368995).

Publikationen

Species	Application	Title
	WB	Proc Natl Acad Sci U S A; Consequences of poly(ethylene oxide) and poloxamer P188 on transcription in healthy and stressed myoblasts; Authors - Adelyn A Crabtree
mouse	WB	Cell Mol Gastroenterol Hepatol; Hepatic TGFβr1 Deficiency Attenuates Lipopolysaccharide/D-Galactosamine-Induced Acute Liver Failure Through Inhibiting GSK3β-Nrf2-Mediated Hepatocyte Apoptosis and Ferroptosis.; Authors - Sha Huang
human	WB	Free Radic Biol Med; High-fat diet aggravates colitis-associated carcinogenesis by evading ferroptosis in the ER stress-mediated pathway.; Authors - Xiaoli Zhang
human	WB	Lung; ETS1 Ameliorates Hyperoxia-Induced Bronchopulmonary Dysplasia in Mice by Activating Nrf2/HO-1 Mediated Ferroptosis; Authors - Min Yang
human	WB	Front Oncol; Development and validation of an endoplasmic reticulum stress-related molecular prognostic model for breast cancer; Authors - Pengyu Fan
human	IHC	J Cell Mol Med; Ferroptosis-related gene NOX4, CHAC1 and HIF1A are valid biomarkers for stomach adenocarcinoma.; Authors - Ruoxi Xiao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Zach (Verified Customer) (08-29-2025)

Pretty great experience with this antibody, gave a prominent and specific band between 25-35kDa in models of acute pancreatitis.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: pancreas, acinar cells