CHAT Polyklonaler Antikörper

CHAT Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20747-1-PBS

Synonyme

CHOACTase, Choline acetylase, choline acetyltransferase, Choline O-acetyltransferase, EC:2.3.1.6

---

• 

  WB analysis using 20747-1-AP (same clone as 20747-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse placenta using 20747-1-AP (same clone as 20747-1-PBS)

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 20747-1-AP (CHAT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IP experiment of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

  IP result of anti-CHAT (IP:20747-1-AP, 4ug; Detection:20747-1-AP 1:500) with rat brain tissue lysate 4000ug. This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 20747-1-AP (same clone as 20747-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20747-1-AP (CHAT antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 20747-1-AP (same clone as 20747-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Neuro-2a using 20747-1-AP (same clone as 20747-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using CHAT antibody (20747-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20747-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

20747-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA CHAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: choline acetyltransferase
• Berechnetes Molekulargewicht: 83 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa, 66-70 kDa
• GenBank-Zugangsnummer: NM_020549
• Gene symbol: CHAT
• Gene ID (NCBI): 1103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. Human CHAT gene generates multiple mRNA splice variants (PMID: 10861222). All these variants possess start codons for translation of 70 kDa cChAT protein (isoform 1). The M variant has an additional start codon for 83 kDa cChAT protein (isoform 2), which has been found in nuclei of neuronal cells in human CNS; and the S variant has another codon for 74 kDa cChAT protein (isoform 3). Besides, CHAT includes a short exon-skipping splice variant (peripheral type CHAT) pCHAT encoding 40-55 kDa (PMID: 31040770, 29759072). This antibody can recognize both pCHAT and cCHAT.