CHAT Polyklonaler Antikörper

CHAT Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24418-1-PBS

Synonyme

---

• 

  WB analysis of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 24418-1-AP (same clone as 24418-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24418-1-AP (CHAT Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IP experiment of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  IP result of anti-CHAT (IP:24418-1-AP, 4ug; Detection:24418-1-AP 1:2000) with rat brain tissue lysate 1880 ug. This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 24418-1-AP (CHAT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24418-1-AP (CHAT Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse brain using 24418-1-AP (same clone as 24418-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 24418-1-AP (CHAT antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24418-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

24418-1-PBS bindet in WB, IHC, IF-P, IP, ELISA CHAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHAT fusion protein Ag19550
• Vollständiger Name: choline acetyltransferase
• Berechnetes Molekulargewicht: 748 aa, 83 kDa
• Beobachtetes Molekulargewicht: 60-66 kDa, 45 kDa
• GenBank-Zugangsnummer: BC130617
• Gene symbol: CHAT
• Gene ID (NCBI): 1103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons. It has been observed that CHAT gene generates multiple mRNA splice variants, including peripheral type CHAT (pCHAT) and common type CHAT (cCHAT), encoding 40-55 kDa and 66-70 kDa forms of proteins, respectively.