CHAT Monoklonaler Antikörper

CHAT Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F7A9

Kat-Nr. : 66816-1-PBS

Synonyme

EC:2.3.1.6, Choline O-acetyltransferase, choline acetyltransferase, Choline acetylase, CHOACTase

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  WB analysis using 66816-1-Ig (same clone as 66816-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-12 using 66816-1-Ig (same clone as 66816-1-PBS)

  PC-12 cells were subjected to SDS PAGE followed by western blot with 66816-1-Ig (CHAT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat small intestine using 66816-1-Ig (same clone as 66816-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 66816-1-Ig (CHAT antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66816-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66816-1-PBS bindet in WB, IHC, Indirect ELISA CHAT und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHAT fusion protein Ag19602
• Vollständiger Name: choline acetyltransferase
• Berechnetes Molekulargewicht: 748 aa, 83 kDa
• Beobachtetes Molekulargewicht: 67-71 kDa
• GenBank-Zugangsnummer: BC130617
• Gene symbol: CHAT
• Gene ID (NCBI): 1103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHAT (choline acetyltransferase) belongs to the carnitine/choline acetyltransferase family. It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA). CHAT is known to to be present exclusively in the cytoplasm of cholinergic neurons and anti-CHAT antibody has been considered as the best marker for cholinergic neurons.