CHCHD10 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHCHD10 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, CoIP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(24)

Kat-Nr. : 25671-1-AP

Synonyme

CoraLite® Plus 488-conjugated CHCHD10 Polyclonal antibody, Protein N27C7-4, Protein N27C7 4, N27C7 4, Coiled-coil-helix-coiled-coil-helix domain-containing protein 10, mitochondrial

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  WB analysis of HAP1 using 25671-1-AP

  HAP1 (WT and CHCHD10 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 25671-1-AP incubated at 1:1000 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of HEK-293 using 25671-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 25671-1-AP

  WB result of CHCHD10 antibody (25671-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD10 transfected HEK-293 cells.

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  WB analysis using 25671-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 25671-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 25671-1-AP (CHCHD10 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 25671-1-AP

  IP result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:25671-1-AP 1:600) with mouse heart tissue lysate 4000ug.

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  IP experiment of HAP1 using 25671-1-AP

  HAP1 lysates prepared and IP of CHCHD10 performed using 1.0 μg of 25671-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  IHC staining of human kidney using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25671-1-AP (CHCHD10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25671-1-AP (CHCHD10 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 25671-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25671-1-AP (CHCHD10 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 25671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 25671-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHCHD10 antibody (25671-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HAP1 using 25671-1-AP

  HAP1 WT cells (yellow outline) and CHCHD10 KO cells (red outline) labelled with a green or a far-red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 25671-1-AP at 1:300 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  CoIP experiment of HepG2 using 25671-1-AP

  Co-IP result of anti-CHCHD10 (IP:25671-1-AP, 4ug; Detection:19424-1-AP 1:600) with HepG2 cells lysate 1400ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Maushirngewebe, Mausherzgewebe, Rattenherzgewebe
• Erfolgreiche IP: Mausherzgewebe, HAP1 cells
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HAP1
• Erfolgreiche Detektion in CoIP: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800
• COImmunpräzipitation (IP): COIP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 8 publications below
• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• IP: See 1 publications below

Produktinformation

25671-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CHCHD10 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Huhn, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHCHD10 fusion protein Ag22598
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 10
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC065232
• Gene symbol: CHCHD10
• Gene ID (NCBI): 400916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD10 is a mitochondrial protein that is enriched at cristae junctions in the intermembrane space and is likely involved in mitochondrial genome stability and maintenance of cristae junctions. Mutations in CHCHD10 have recently been described as a cause of frontotemporal dementia (FTD) comorbid with amyotrophic lateral sclerosis (ALS).

Publikationen

Species	Application	Title
mouse	WB,IF	Nat Commun; Loss of function CHCHD10 mutations in cytoplasmic TDP-43 accumulation and synaptic integrity.; Authors - Jung-A A Woo ; KD Validated
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
mouse	IHC	J Mol Cell Biol; Single-cell transcriptomes reveal molecular specializations of neuronal cell types in the developing cerebellum.; Authors - Jian Peng
mouse	WB	Diabetes; CHCHD10 Modulates Thermogenesis of Adipocytes by Regulating Lipolysis; Authors - Meng Ding ; KO Validated
mouse	WB	Mol Ther Nucleic Acids; Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson's disease.; Authors - Colleen S Stein
human	WB,IF	Hum Mol Genet; Loss of CHCHD10-CHCHD2 complexes required for respiration underlies the pathogenicity of a CHCHD10 mutation in ALS.; Authors - Isabella R Straub ; KD Validated