CHCHD2 Polyklonaler Antikörper

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• KD/KO Validated

CHCHD2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19424-1-PBS

Synonyme

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  WB analysis using 19424-1-AP (same clone as 19424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 19424-1-AP (same clone as 19424-1-PBS)

  WB result of CHCHD2 antibody (19424-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 19424-1-AP (same clone as 19424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human adrenal gland using 19424-1-AP (same clone as 19424-1-PBS)

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

  IP result of anti-CHCHD2 (IP:19424-1-AP, 4ug; Detection:19424-1-AP 1:1000) with HepG2 cells lysate 2520 ug. This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 19424-1-AP (same clone as 19424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 19424-1-AP (same clone as 19424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 19424-1-AP (same clone as 19424-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19424-1-AP (same clone as 19424-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using CHCHD2 antibody 19424-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19424-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19424-1-PBS bindet in WB, IHC, IF/ICC, IP, ELISA CHCHD2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHCHD2 fusion protein Ag13752
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 2
• Berechnetes Molekulargewicht: 151 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16-18 kDa
• GenBank-Zugangsnummer: BC003079
• Gene symbol: CHCHD2
• Gene ID (NCBI): 51142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).