CHCHD2 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CHCHD2 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G1G10

Kat-Nr. : 66302-1-PBS

Synonyme

2G1G10, AAG10, Aging-associated gene 10 protein, C7orf17, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2

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  WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66302-1-Ig (same clone as 66302-1-PBS)

  WB result of CHCHD2 antibody (66302-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66302-1-Ig (same clone as 66302-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 66302-1-Ig (same clone as 66302-1-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 66302-1-Ig (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66302-1-Ig (same clone as 66302-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66302-1-Ig (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66302-1-Ig (same clone as 66302-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (66302-1-Ig, Clone: 2G1G10 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66302-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66302-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CHCHD2 und zeigt Reaktivität mit Hausschwein, human, Ratten

• Getestete Reaktivität: Hausschwein, human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CHCHD2 fusion protein Ag24219
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 2
• Berechnetes Molekulargewicht: 151 aa, 16 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC003079
• Gene symbol: CHCHD2
• Gene ID (NCBI): 51142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).