CHCHD2 Rekombinanter Antikörper

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• KD/KO Validated

CHCHD2 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

4A12

Rezensionen (1)

Kat-Nr. : 82702-1-RR

Synonyme

HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10

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• 

  WB analysis using 82702-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82702-1-RR (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 82702-1-RR

  WB result of CHCHD2 antibody (82702-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

• 

  IF Staining of HepG2 using 82702-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (82702-1-RR, Clone: 4A12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 82702-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82702-1-RR against Human CHCHD2 were performed. The affinity constant is 2.12 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82702-1-RR bindet in WB, IF/ICC, ELISA CHCHD2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHCHD2 fusion protein Ag13752
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 2
• Berechnetes Molekulargewicht: 151 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC003079
• Gene symbol: CHCHD2
• Gene ID (NCBI): 51142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vignesh (Verified Customer) (08-28-2025)

It's an excellent product with reduced or no nonspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HTB126