CHCHD3/MIC19 Polyklonaler Antikörper

CHCHD3/MIC19 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(35)

Kat-Nr. : 25625-1-AP

Synonyme

CHCHD3, Coiled-coil-helix-coiled-coil-helix domain-containing protein 3, MIC19, MICOS complex subunit MIC19, MINOS3



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHEK-293-Zellen, A431-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mauslebergewebe, NIH/3T3-Zellen
Erfolgreiche IPHeLa-Zellen
Erfolgreiche Detektion in IHChumanes Lebergewebe, humanes Herzgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:4000-1:12000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

25625-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA CHCHD3/MIC19 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CHCHD3/MIC19 fusion protein Ag22579
Vollständiger Name coiled-coil-helix-coiled-coil-helix domain containing 3
Berechnetes Molekulargewicht 227 aa, 26 kDa
Beobachtetes Molekulargewicht 26 kDa
GenBank-ZugangsnummerBC014839
Gene symbol CHCHD3
Gene ID (NCBI) 54927
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for CHCHD3/MIC19 antibody 25625-1-APProtokoll herunterladen
IHC protocol for CHCHD3/MIC19 antibody 25625-1-APProtokoll herunterladenl
IF protocol for CHCHD3/MIC19 antibody 25625-1-APProtokoll herunterladen
IP protocol for CHCHD3/MIC19 antibody 25625-1-APProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
mouseIF

Acta Neuropathol

Mitochondria, ER, and nuclear membrane defects reveal early mechanisms for upper motor neuron vulnerability with respect to TDP-43 pathology.

Authors - Mukesh Gautam
humanWB

Mol Cell

Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.

Authors - Milena Vukotic
mouse,humanWB

Nat Commun

Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA

Authors - Ayesha Sen
humanWB

EMBO Rep

Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.

Authors - Arun Kumar Kondadi
mouseWB

Cell Rep

Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.

Authors - Christina Glytsou

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Mia (Verified Customer) (09-28-2022)

goood!

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: mouse tissue