CHCHD3 Polyklonaler Antikörper

CHCHD3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

24

Rezensionen (1)

Kat-Nr. : 25625-1-AP

Synonyme

CHCHD3, MIC19, MINOS3

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Galerie der Validierungsdaten

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  WB analysis using 25625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 25625-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 25625-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 25625-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 25625-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25625-1-AP (CHCHD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25625-1-AP

  IP Result of anti-CHCHD3 (IP:25625-1-AP, 4ug; Detection:25625-1-AP 1:1000) with HeLa cells lysate 2600ug.

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  IHC staining of human liver using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25625-1-AP (CHCHD3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25625-1-AP (CHCHD3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human heart using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 25625-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 25625-1-AP (CHCHD3 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 25625-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (25625-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HepG2 using 25625-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD3 antibody (25625-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, A431-Zellen, HeLa-Zellen, humanes Plazenta-Gewebe, Jurkat-Zellen, Mauslebergewebe, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:4000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 22 publications below
• IF: See 2 publications below

Produktinformation

25625-1-AP bindet in WB, IP, IHC, IF, ELISA CHCHD3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHCHD3 fusion protein Ag22579
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 3
• Berechnetes Molekulargewicht: 227 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC014839
• Gene symbol: CHCHD3
• Gene ID (NCBI): 54927
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHCHD3, initially identified as a substrate for cAMP-dependent protein kinase (PKA), is a ubiquitous protein in the mitochondria and plays a prominent role in maintaining cristae integrity and mitochondrial function. In mitochondria, ChChd3 is predominantly localized to the inner membrane (IM), facing toward the intermembrane space (IMS), and is part of the large protein complex now called as MINOS (mitochondrial inner membrane organizing system), or MIB (mitochondrial intermembrane space bridging) CHCHD3 is highly conserved in mammals with human and mouse protein sharing ∼92% sequence similarity.

Publikationen

Species	Application	Title
mouse	IF	Acta Neuropathol; Mitochondria, ER, and nuclear membrane defects reveal early mechanisms for upper motor neuron vulnerability with respect to TDP-43 pathology.; Authors - Mukesh Gautam
human	WB	Mol Cell; Acylglycerol Kinase Mutated in Sengers Syndrome Is a Subunit of the TIM22 Protein Translocase in Mitochondria.; Authors - Milena Vukotic
mouse,human	WB	Nat Commun; Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA; Authors - Ayesha Sen
human	WB	EMBO Rep; Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.; Authors - Arun Kumar Kondadi
mouse	WB	Cell Rep; Optic Atrophy 1 Is Epistatic to the Core MICOS Component MIC60 in Mitochondrial Cristae Shape Control.; Authors - Christina Glytsou
human	WB	Sci Rep; Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.; Authors - Chengli Ding

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mia (Verified Customer) (09-28-2022)

goood！

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue

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