CHCHD6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHCHD6 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(15)

Rezensionen (4)

Kat-Nr. : 20639-1-AP

Synonyme

MICOS complex subunit MIC25, MIC25, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, Coiled-coil-helix cristae morphology protein 1, CHCM1

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  WB analysis using 20639-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20639-1-AP (CHCHD6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 20639-1-AP

  IP result of anti-CHCHD6 (IP:20639-1-AP, 4ug; Detection:20639-1-AP 1:2000) with Jurkat cells lysate 1560 ug.

• 

  IHC staining of human stomach using 20639-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20639-1-AP (CHCHD6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human stomach using 20639-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 20639-1-AP (CHCHD6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 20639-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CHCHD6 antibody (20639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IF: See 1 publications below

Produktinformation

20639-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA CHCHD6 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHCHD6 fusion protein Ag14700
• Vollständiger Name: coiled-coil-helix-coiled-coil-helix domain containing 6
• Berechnetes Molekulargewicht: 235 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-29 kDa
• GenBank-Zugangsnummer: BC006123
• Gene symbol: CHCHD6
• Gene ID (NCBI): 84303
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Elife; QIL1 is a novel mitochondrial protein required for MICOS complex stability and cristae morphology.; Authors - Virginia Guarani
human	WB	EMBO Rep; Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.; Authors - Arun Kumar Kondadi
human	WB	Elife; QIL1 mutation causes MICOS disassembly and early onset fatal mitochondrial encephalopathy with liver disease.; Authors - Virginia Guarani
mouse	WB	Cell Death Differ; Mic60/Mitofilin determines MICOS assembly essential for mitochondrial dynamics and mtDNA nucleoid organization.; Authors - H Li ; KD Validated
human	WB	Sci Rep; Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure.; Authors - Chengli Ding ; KO Validated
human	WB	Biochim Biophys Acta Biomembr; Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.; Authors - Jennifer Urbach

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Angela (Verified Customer) (12-27-2024)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart tissue

CHCHD6 Antibody Western Blot validation (1:1000 dilution) in heart tissue (Cat no:20639-1-AP)

FH

angela (Verified Customer) (12-27-2024)

very good antibody

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000
• Cell Tissue Type: heart tissue

CHCHD6 Antibody Western Blot validation (1;1000 dilution) in heart tissue (Cat no:20639-1-AP)

FH

SITING (Verified Customer) (07-13-2020)

very clear band in our wester blot expose result

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MOUSE HEART

FH

Jingwen (Verified Customer) (01-28-2020)

This antibody worked very well when I performed western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Cancer tissue