CHD2 Polyklonaler Antikörper

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• KD/KO Validated

CHD2 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21334-1-PBS

Synonyme

EC:3.6.4.12, Chromodomain-helicase-DNA-binding protein 2, CHD-2, CHD 2, ATP-dependent helicase CHD2

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  WB analysis using 21334-1-AP (same clone as 21334-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21334-1-AP (CHD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 21334-1-AP (same clone as 21334-1-PBS)

  WB result of CHD2 antibody (21334-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHD2 transfected HEK-293 cells. This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 21334-1-AP (same clone as 21334-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21334-1-AP (CHD2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21334-1-AP (same clone as 21334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21334-1-AP (same clone as 21334-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD2 antibody (21334-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 21334-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21334-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CHD2 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHD2 fusion protein Ag15429
• Vollständiger Name: chromodomain helicase DNA binding protein 2
• Berechnetes Molekulargewicht: 1828 aa, 211 kDa
• Beobachtetes Molekulargewicht: 230-250 kDa
• GenBank-Zugangsnummer: BC020810
• Gene symbol: CHD2
• Gene ID (NCBI): 1106
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromodomain helicase DNA binding protein 2 (CHD2), a member of the sucrose nonfermenting (SNF-2) protein family of epigenetic regulators, which use the energy from ATP hydrolysis to change nucleosome positioning, composition and chromatin structure(PMID: 33435571). CHD2 protein plays a critical role in embryonic development, tumor suppression and survival(PMID: 24834135). In addition, CHD2 has been proposed to prevent breast cancer initiation, and CHD2 mutations are associated with chronic lymphocytic leukemia.