CHD7 Polyklonaler Antikörper

CHD7 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31919-1-PBS

Synonyme

ATP-dependent helicase CHD7, CHD-7, Chromodomain Helicase DNA Binding Protein 7, Chromodomain-helicase-DNA-binding protein 7, EC:3.6.4.12

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  WB analysis using 31919-1-AP (same clone as 31919-1-PBS)

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 31919-1-AP (CHD7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IHC staining of rat cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 31919-1-AP (same clone as 31919-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 31919-1-AP (CHD7 antibody) at dilution of 1:1500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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  IF Staining of HEK-293 using 31919-1-AP (same clone as 31919-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CHD7 antibody (31919-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 31919-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31919-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CHD7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHD7 fusion protein Ag36595
• Vollständiger Name: chromodomain helicase DNA binding protein 7
• Beobachtetes Molekulargewicht: 350 kDa
• GenBank-Zugangsnummer: BC110818
• Gene symbol: CHD7
• Gene ID (NCBI): 55636
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromodomain helicase DNA-binding protein 7 (CHD7) is an ATP-dependent eukaryotic chromatin remodeling enzyme that regulates nucleosome positioning and alters DNA accessibility, and is essential for organ development.CHD7 is a gene known to be associated with CHARGE syndrome, Kallmann syndrome, and hypogonadotropic hypogonadism, where it is associated with CHARGE syndrome is a congenital multiorgan disorder characterized by eye defects, heart defects, posterior nasal atresia, growth retardation, genital anomalies, ear malformations, and deafness. The effects of CHD7 mutations on inner ear development, neuronal differentiation, cardiovascular development, and regulation of bone lipid homeostasis have been studied.