CHD8 Rekombinanter Antikörper

CHD8 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250612C4

Kat-Nr. : 86032-1-RR

Synonyme

CHD 8, HELSNF1

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  WB analysis using 86032-1-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 86032-1-RR (CHD8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 86032-1-RR

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 86032-1-RR (CHD8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86032-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86032-1-RR (CHD8 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86032-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CHD8 antibody (86032-1-RR, Clone: 250612C4 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, Jurkat-Zellen, MCF-7-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86032-1-RR bindet in WB, IF/ICC, ELISA CHD8 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHD8 fusion protein Ag31425
• Vollständiger Name: chromodomain helicase DNA binding protein 8
• Berechnetes Molekulargewicht: 290KD
• Beobachtetes Molekulargewicht: 290 kDa
• GenBank-Zugangsnummer: NM_001170629
• Gene symbol: CHD8
• Gene ID (NCBI): 57680
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromodomain helicase domain 8 (CHD8) is one of the most frequently mutated and most penetrant genes in the autism spectrum disorder (ASD). CHD8 is located on 14q11.2. It is part of the SNF2H-like ATP-dependent chromatin remodeling enzymes family referred to as CHD (chromodomain helicase DNA binding). CHD8 has two isoforms: CHD8L, a full-length protein of 280 kDa; and CHD8S (Duplin), a 110 kDa protein of the NH2-terminal chromodomain region resulting from alternative splicing (PMID:19151705). CHD8 is essential for development, as homozygote mutant mice die at an embryonic stage. CHD8 is expressed in the mouse at the embryonic stage (E12.5) in different levels in wide regions of the brain (neocortex, forebrain, ventricular, subventricular and mantle zones, rhombic lip (RL), and the isthmus of the cerebellum, as well as in lower RL and floor plate region of the hindbrain, midbrain, diencephalon, hypothalamus, pituitary gland, craniofacial region, and tongue and olfactory epithelium). In the postnatal mouse brain (P20), CHD8 is expressed in the cerebellum, neocortex, hippocampus, hypothalamus, and olfactory bulb (PMID:30277262). Peak expression levels were observed at E18-P7, then gradually decreased to adulthood. Highest expression was found in neurons, and lower levels in astrocyte and astroglia (PMID:30574290). In the mouse, CHD8 is expressed higher in brain compared to other tissues and in the embryo, compared to adult.