CHD9 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CHD9 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13402-1-PBS

Synonyme

AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator

---

• 

  WB analysis using 13402-1-AP (same clone as 13402-1-PBS)

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 13402-1-AP (same clone as 13402-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 13402-1-AP (same clone as 13402-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

• 

  IF Staining of Hela using 13402-1-AP (same clone as 13402-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using CHD9 antibody (13402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 13402-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13402-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CHD9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHD9 fusion protein Ag4245
• Vollständiger Name: chromodomain helicase DNA binding protein 9
• Berechnetes Molekulargewicht: 2897 aa, 326 kDa
• Beobachtetes Molekulargewicht: 330 kDa
• GenBank-Zugangsnummer: BC033770
• Gene symbol: CHD9
• Gene ID (NCBI): 80205
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).