Chromogranin A Monoklonaler Antikörper

Chromogranin A Monoklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

10

CloneNo.

3H11C11

Kat-Nr. : 60135-1-Ig

Synonyme

CGA, CHGA, Chromogranin A, Pituitary secretory protein I, SP I, Vasostatin I Vasostatin 2

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Galerie der Validierungsdaten

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  IHC staining of human pancreas using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:80000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:80000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60135-1-Ig(Chromogranin A antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon using 60135-1-Ig(Chromogranin A antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human lung cancer using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 60135-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of PC-12 using 60135-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using 60135-1-Ig (Chromogranin A antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Pankreasgewebe, humanes Kolongewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-12-Zellen

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:5000-1:80000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 5 publications below

Produktinformation

60135-1-Ig bindet in WB, IHC, IF, FC, ELISA Chromogranin A und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Chromogranin A fusion protein Ag0807
• Vollständiger Name: chromogranin A (parathyroid secretory protein 1)
• Berechnetes Molekulargewicht: 51 kDa
• GenBank-Zugangsnummer: BC006459
• Gene symbol: CHGA
• Gene ID (NCBI): 1113
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin A is a member of the granin family of neuroendocrine secretory proteins. It is located in secretory vesicles of neurons and endocrine cells. Chromogranin A is the precursor to several functional peptides including vasostatin, pancreastatin, catestatin and parastatin. These peptides negatively modulate the neuroendocrine function of the releasing cell (autocrine) or nearby cells (paracrine). CgA is one of the most used tumor markers in NET's (neuroendocrine tumors) , and elevated CgA concentrations have been demonstrated in serum or plasma of patients with different types of these tumors.

Publikationen

Species	Application	Title
mouse	IF	Mol Metab; Genetic activation of α-cell glucokinase in mice causes enhanced glucose-suppression of glucagon secretion during normal and diabetic states.; Authors - Varun Bahl
human	WB,IHC	J Pathol; Histone demethylase PHF8 drives neuroendocrine prostate cancer progression by epigenetically upregulating FOXA2.; Authors - Qiuli Liu
	IF	iScience; Efficient Generation and Transcriptomic Profiling of Human iPSC-Derived Pulmonary Neuroendocrine Cells.; Authors - Pooja Hor
	WB	Sci Rep; PMID: 31197210; Authors - Trine Maxel
human	WB	Sci Rep; Cancer cell's neuroendocrine feature can be acquired through cell-cell fusion during cancer-neural stem cell interaction.; Authors - Liyuan Yin
human	IF	Transl Oncol; High throughput proteomic and metabolic profiling identified target correction of metabolic abnormalities as a novel therapeutic approach in head and neck paraganglioma.; Authors - Zhigang Wang