CHGB Rekombinanter Antikörper

CHGB Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242952C9

Kat-Nr. : 85438-4-PBS

Synonyme

CgB, Chromogranin B, SCG1, 242952C9, Chromogranin-B

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  WB analysis using 85438-4-RR (same clone as 85438-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

• 

  WB analysis using 85438-4-RR (same clone as 85438-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85438-4-RR (CHGB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 85438-4-RR (same clone as 85438-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85438-4-RR (CHGB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 85438-4-RR (same clone as 85438-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 85438-4-RR (CHGB antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85438-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85438-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85438-4-RR against Human CHGB were performed. The affinity constant is 1.95 nM.

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Geprüfte Anwendungen

Produktinformation

85438-4-PBS bindet in WB, IHC, Indirect ELISA CHGB und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CHGB fusion protein Ag6833
• Vollständiger Name: chromogranin B (secretogranin 1)
• Berechnetes Molekulargewicht: 78 kDa
• Beobachtetes Molekulargewicht: 70-100 kDa
• GenBank-Zugangsnummer: BC000375
• Gene symbol: Chromogranin B
• Gene ID (NCBI): 1114
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa.