CHIA Polyklonaler Antikörper

CHIA Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 21484-1-AP

Synonyme

TSA1902, Lung-specific protein TSA1902, Lung specific protein TSA1902, EC:3.2.1.14, AMCase

---

• 

  WB analysis of mouse lung using 21484-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21484-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21484-1-AP (CHIA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 21484-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach using 21484-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 21484-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 21484-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 21484-1-AP (CHIA antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 21484-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 21484-1-AP

  Immunofluorescent analysis of Hela cells, using CHIA antibody 21484-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslungengewebe
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Lungengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen, HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IF: See 2 publications below

Produktinformation

21484-1-AP bindet in WB, IHC, IF/ICC, ELISA CHIA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CHIA fusion protein Ag15767
• Vollständiger Name: chitinase, acidic
• Berechnetes Molekulargewicht: 476 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC036339
• Gene symbol: CHIA
• Gene ID (NCBI): 27159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Front Mol Biosci; Characterization of an Aging-Based Diagnostic Gene Signature and Molecular Subtypes With Diverse Immune Infiltrations in Atherosclerosis.; Authors - Lei Zhao
mouse	IF	Commun Biol; Elevated stress response marks deeply quiescent reserve cells of gastric chief cells; Authors - Daisuke Shiokawa
mouse	WB,IF	Eur J Immunol; Aspergillus fumigatus corneal infection is regulated by chitin synthases and by neutrophil-derived acidic mammalian chitinase.; Authors - Steven de Jesus Carrion