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Chk1 Rekombinanter Antikörper

Chk1 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2G7

Kat-Nr. : 80056-2-PBS

Synonyme

CHEK1, 2G7, Checkpoint kinase-1, CHEK 1, Chk 1

Filter:
  • Western Blot (WB) analysis of various lysates using Chk1 Recombinant antibody (80056-2-RR)

    WB analysis using 80056-2-RR (same clone as 80056-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80056-2-RR (Chk1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80056-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293T cells using Chk1 Recombinant antibody (80056-2-RR)

    IF Staining of HEK-293T using 80056-2-RR (same clone as 80056-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293T cells using Chk1 antibody (80056-2-RR, Clone: 2G7 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80056-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

80056-2-PBS bindet in WB, IF/ICC, Indirect ELISA Chk1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Peptid
Vollständiger Name CHK1 checkpoint homolog (S. pombe)
Berechnetes Molekulargewicht 54 kDa
Beobachtetes Molekulargewicht 55 kDa
GenBank-ZugangsnummerBC004202
Gene symbol Chk1
Gene ID (NCBI) 1111
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CHEK1(Checkpoint kinase-1) is also named as CHK1 and belongs to the protein kinase superfamily. It is implicated in a circuit in which it activates checkpoints, DNA repair and proliferating cell nuclear antigen and FANCD2 monoubiquitinylation(PMID:21389083). CHEK1 protects vertebrate cells against spontaneous chromosome missegregation and is required to sustain anaphase delay when spindle function is disrupted by taxol(PMID:17276342). It has 3 isoforms produced by alternative splicing with the molecular mass of 54 kDa, 44 kDa and 50 kDa.