Choline Kinase Alpha Rekombinanter Antikörper

Choline Kinase Alpha Rekombinant Antikörper für WB, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242095B4

Kat-Nr. : 84881-2-PBS

Synonyme

CHKA, 242095B4, CHETK-alpha, CHK, CKI

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  WB analysis using 84881-2-RR (same clone as 84881-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84881-2-RR (CHKA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84881-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01644-2

  Cytometric bead array standard curve of MP01644-2, CHKA Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84881-1-PBS. Detection antibody: 84881-2-PBS. Standard: Ag4761. Range: 1.563-200 ng/mL.

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  Affinity and Kinetic Characterization of 84881-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84881-2-RR against Human CHKA were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84881-2-PBS bindet in WB, Cytometric bead array, Indirect ELISA Choline Kinase Alpha und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Choline Kinase Alpha fusion protein Ag4761
• Vollständiger Name: choline kinase alpha
• Berechnetes Molekulargewicht: 439 aa, 52 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC036471
• Gene symbol: CHKA
• Gene ID (NCBI): 1119
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Choline kinase alpha, also named as CHK, CKI and CK, belongs to the choline/ethanolamine kinase family. CHKa may have a regulatory role in phosphatidylcholine synthesis. CHKA catalyze the reaction: ATP + choline = ADP + O-phosphocholine. The human enzyme resembled the rat liver enzyme over the entire sequence. It also resembled the yeast enzyme in the carboxy-terminal region, but not in the amino-terminal region(PMID:1618328).And it expresses in human brain tissue but not in human skeletal muscle(PMID:21665002).It has 2 isoforms produced by alternative splicing.